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What is Colorimetric Analysis of Reducing Sugar about?

This lab manual outlines two colorimetric methods for determining reducing sugar concentration - the Somogyi Nelson method and the DNS method. The Somogyi Nelson method involves reacting the sugar sample with copper and arsenite reagents, then measuring absorbance. The DNS method involves boiling the sugar sample with 3,5-dinitrosalicylic acid reagent and then measuring absorbance. Standard curves are made using known glucose concentrations to quantify the unknown sample concentrations. Students are instruc

Author

Msfaeza Hanafi

Language

EN