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An extremely sensitive species-specific ARMs PCR test for the presence of tiger bone DNA by Jon H. Wetton; Carol S.F. Tsang; Chris A. Roney; Adrian C. Spriggs is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is An extremely sensitive species-specific ARMs PCR test for the presence of tiger bone DNA about?

The survival of the tiger (Panthera tigris) is seriously threatened by poaching to provide raw materials for Traditional Chinese Medicines (TCMs). Most highly prized are the tiger's bones, which are used in combination with other animal and plant derivatives in pills and plasters for the treatment of rheumatism and other ailments. Hundreds of patent remedies have been produced which claim to contain tiger bone, but proof of its presence is needed, if legislation prohibiting the trade in endangered species is to be enforced. A highly sensitive tiger-specific real-time PCR assay has been developed to address this problem. Using primers specific to the tiger mitochondrial cytochrome b gene, successful amplification has been reliably achieved from blood, hair and bone as well as from a range of TCMs spiked with 0.5% tiger bone. Although capable of detecting fewer than 10 substrate molecules, the seven varieties of TCM pills and plasters tested showed no detectable trace of tiger DNA before spiking. Furthermore, sequencing several ''tiger bone'' fragments seized from TCM shops has shown that they actually originated from cattle and pigs. The potential effects of traditional bone prepara

Who reads An extremely sensitive species-specific ARMs PCR test for the presence of tiger bone DNA?

Author

Jon H. Wetton; Carol S.F. Tsang; Chris A. Roney; Adrian C. Spriggs

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0379-0738)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)