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Multi-locus sequence typing of Ehrlichia ruminantium strains from geographically diverse origins and collected in Amblyomma variegatum from Uganda by Ryo Nakao; Joseph W Magona; Lijia Zhou; Frans Jongejan; Chihiro Sugimoto is a Medicine article available to read on EtoBox.

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## Background The rickettsial bacterium __Ehrlichia ruminantium__ is the causative agent of heartwater in ruminants. A better understanding of the population genetics of its different strains is, however, needed for the development of novel diagnostic tools, therapeutics and prevention strategies. Specifically, the development of effective vaccination policies relies on the proper genotyping and characterisation of field isolates. Although multi-locus sequence typing (MLST) has been recently developed, only strains from geographically restricted collections have been analysed so far. The expansion of the MLST database to include global strains with different geographic origins is therefore essential. In this study, we used a panel of reference strains from geographically diverse origins and field samples of __E. ruminantium__ detected from its vector, __Amblyomma variegatum__, in heartwater-endemic areas in Uganda. ## Results A total of 31 novel alleles (six, four, six, three, two, five, three, and two for __gltA, groEL, lepA, lipA, lipB, secY, sodB__, and __sucA__ loci, respectively) and 19 novel sequence types (STs) were identified. Both neighbour-joining and minimum spanning tre

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Author

Ryo Nakao; Joseph W Magona; Lijia Zhou; Frans Jongejan; Chihiro Sugimoto

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2008-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Research Square (ISSN 1756-3305)

Published

2011

Language

EN

Field

Medicine (Health Sciences)