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Human antibodies targeting a Mycobacterium transporter protein mediate protection against tuberculosis by Watson, Avia (author);Li, Hao (author);Ma, Bingting (author);Weiss, Ronen (author);Bendayan, Daniele (author);Abramovitz, Lilach (author);Ben-Shalom, Noam (author);Mor, Michael (author);Pinko, Erica (author);Bar Oz, Michal (author);Wang, Zhenqi (author);Du, Fengjiao (author);Lu, Yu (author);Rybniker, Jan (author);Dahan, Rony (author);Huang, Hairong (author);Barkan, Daniel (author);Xiang, Ye (author);Javid, Babak (author);Freund, Natalia T. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Human antibodies targeting a Mycobacterium transporter protein mediate protection against tuberculosis about?

## Abstract Mycobacterium tuberculosis (Mtb) exposure drives antibody responses, but whether patients with active tuberculosis elicit protective antibodies, and against which antigens, is still unclear. Here we generate monoclonal antibodies from memory B cells of one patient to investigate the B cell responses during active infection. The antibodies, members of four distinct B cell clones, are directed against the Mtb phosphate transporter subunit PstS1. Antibodies p4-36 and p4-163 reduce Mycobacterium bovis-BCG and Mtb levels in an ex vivo human whole blood growth inhibition assay in an FcR-dependent manner; meanwhile, germline versions of p4-36 and p4-163 do not bind Mtb. Crystal structures of p4-36 and p4-170, complexed to PstS1, are determined at 2.1 Å and 2.4 Å resolution, respectively, to reveal two distinctive PstS1 epitopes. Lastly, a prophylactic p4-36 and p4-163 treatment in Mtb-infected Balb/c mice reduces bacterial lung burden by 50%. Our study shows that inhibitory anti-PstS1 B cell responses arise during active tuberculosis.

Who reads Human antibodies targeting a Mycobacterium transporter protein mediate protection against tuberculosis?

It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.

Author
Watson, Avia (author);Li, Hao (author);Ma, Bingting (author);Weiss, Ronen (author);Bendayan, Daniele (author);Abramovitz, Lilach (author);Ben-Shalom, Noam (author);Mor, Michael (author);Pinko, Erica (author);Bar Oz, Michal (author);Wang, Zhenqi (author);Du, Fengjiao (author);Lu, Yu (author);Rybniker, Jan (author);Dahan, Rony (author);Huang, Hairong (author);Barkan, Daniel (author);Xiang, Ye (author);Javid, Babak (author);Freund, Natalia T. (author)
Publisher
Springer Science and Business Media LLC
Published
2021
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)

More by Watson, Avia (author);Li, Hao (author);Ma, Bingting (author);Weiss, Ronen (author);Bendayan, Daniele (author);Abramovitz, Lilach (author);Ben-Shalom, Noam (author);Mor, Michael (author);Pinko, Erica (author);Bar Oz, Michal (author);Wang, Zhenqi (author);Du, Fengjiao (author);Lu, Yu (author);Rybniker, Jan (author);Dahan, Rony (author);Huang, Hairong (author);Barkan, Daniel (author);Xiang, Ye (author);Javid, Babak (author);Freund, Natalia T. (author)

Browse all works by Watson, Avia (author);Li, Hao (author);Ma, Bingting (author);Weiss, Ronen (author);Bendayan, Daniele (author);Abramovitz, Lilach (author);Ben-Shalom, Noam (author);Mor, Michael (author);Pinko, Erica (author);Bar Oz, Michal (author);Wang, Zhenqi (author);Du, Fengjiao (author);Lu, Yu (author);Rybniker, Jan (author);Dahan, Rony (author);Huang, Hairong (author);Barkan, Daniel (author);Xiang, Ye (author);Javid, Babak (author);Freund, Natalia T. (author)