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Weaver cerebellar granule neurons show altered expression of NMDA receptor subunits both in vivo and in vitro by Päivi Liesi; Randall R. Stewart; B. Emmanuel Akinshola; Jerry M. Wright is a Neuroscience article available to read on EtoBox.

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Biochemical, immunocytochemical, and molecular biological techniques were used to investigate the expression of N-methyl-D-aspartate (NMDA) receptor subunits in migration-deficient weaver mouse cerebellum in vivo and in primary cultures of the vermal weaver granule neurons with or without a rescue by verapamil. We found that both NMDAR1(1) message and protein were expressed by the weaver granule neurons in situ. Immunocytochemical and biochemical analyses indicated that granule neurons of the weaver cerebellum expressed R1(1) and R2A(⑀1) subunits but showed little expression of the R2B(⑀2) subunit. In weaver cerebellum, the R2B(⑀2) subunit was primarily expressed in nerve fibers of the internal granule cell layer and white matter. Reverse-transcriptase-polymerase chain reaction followed by sequence analysis of the R1(1) subunit indicated that the 1 subunit amplicons of both normal and weaver cerebella were identical, and that splice variants with exon 22 (1-2) and with or without exon 5 (a/b) or exon 21 (1-4) were detectable. The R2A(⑀1), and R2B(⑀2) subunits of the normal and weaver mouse cerebellum revealed no primary structural differences between the normal and weaver NMDA rece

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Author

Päivi Liesi; Randall R. Stewart; B. Emmanuel Akinshola; Jerry M. Wright

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3034)

Published

1999

Language

EN

Field

Neuroscience (Life Sciences)