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This document discusses an experiment to isolate and count microorganisms from a soil sample using dilution and aseptic techniques. Serial dilution is used to reduce the concentration of bacteria to a feasible level for counting colonies. Viable titre is determined from colony forming units and dilution factor. Sodium chloride is added as a selective agent to inhibit bacteria that cannot tolerate hypertonic conditions and remove water from cells, reducing the number of colonies compared to isolation without

Author

Darzeman Raize

Language

EN