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Temperature dependence of nanosecond charge recombination in mutant Rhodobacter sphaeroides reaction centers: modelling of the protein dynamics by Krzysztof Gibasiewicz; Maria Pajzderska; Rafał Białek; Michael R. Jones is a Materials Science article available to read on EtoBox.

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## Abstract We investigated the influence of a range of factors—temperature, redox midpoint potential of an electron carrier, and protein dynamics—on nanosecond electron transfer within a protein. The model reaction was back electron transfer from a bacteriopheophytin anion, H~A~^−^, to an oxidized primary electron donor, P^+^, in a wild type Rhodobacter sphaeroides reaction center (RC) with a permanently reduced secondary electron acceptor (quinone, Q~A~^−^). Also used were two modified RCs with single amino acid mutations near the monomeric bacteriochlorophyll, B~A~, located between P and H~A~. Both mutant RCs showed significant slowing down of this back electron transfer reaction with decreasing temperature, similar to that observed with the wild type RC, but contrasting with a number of single point mutant RCs studied previously. The observed similarities and differences are explained in the framework of a (P^+^B~A~^−^ ↔ P^+^H~A~^−^) equilibrium model with an important role played by protein relaxation. The major cause of the observed temperature dependence, both in the wild type RC and in the mutant proteins, is a limitation in access to the thermally activated pathway of char

Who reads Temperature dependence of nanosecond charge recombination in mutant Rhodobacter sphaeroides reaction centers: modelling of the protein dynamics?

Author

Krzysztof Gibasiewicz; Maria Pajzderska; Rafał Białek; Michael R. Jones

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Materials Science (Physical Sciences)