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Untersuchungen zum Schlpfen der Miracidien des Kleinen Leberegels Dicrocoelium dendriticum by Karl-Eike Mitterer is a Immunology and Microbiology article available to read on EtoBox.

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## Studies on the Hatching oB Miracidia of Dicrocoelium dendriticum Summary. The eggs of Dicrocoelium dendriticum were induced to open by solutions of formie acid and caproic acid (Table 1). The miracidia hatched in O2-free water after the eggs had been dried with H 2 or in vacuum. The miracidia were able to live for 3 honrs if water contained 20 m~ HaC1, 10 mM KC1, and 1 mM CaCl 2. Ca++-ions are obviously necessary for the mobility of miracidia. The experimental use of intestinal juice of the Roman snail Helix pomatia gave hatching results which were dependent on the absence of 02 (exposure to H2) and the presence of baeteria with a still unknown function. The dependence on pH seems to be indirect (Abb. 2). Studies on the permeabilities of the egg shell and the embryonic membr~ne ("vitelline membrane"), the evidence of an oligosaccharide (Abb. 3) liberated from the "spaltraum" (Abb. 1) during egg-opening, and the determination of the osmotie pressure of the hatching process (50 % hatching in 1.2--1.4 Osmols sucrose/1000 ml H20; Abb. 4) led to the following hypothesis of hatching mechanism. After the activation of the granular gland of the miracidium an enzyme is released into the

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Author

Karl-Eike Mitterer

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1432-1955)

Published

1975

Language

DE

Field

Immunology and Microbiology (Life Sciences)