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Separation of Lactose from Human Milk Oligosaccharides with Simulated Moving Bed Chromatography by Andreas Geisser; Tatjana Hendrich; Günther Boehm; Bernd Stahl is a Chemistry article available to read on EtoBox.

What is Separation of Lactose from Human Milk Oligosaccharides with Simulated Moving Bed Chromatography about?

The successful separation of the disaccharide lactose from a complex mixture of human milk oligosaccharides (HMOS) with the continuous chromatography of simulated moving bed (SMB) technique is described. Since lactose is the main carbohydrate in human milk with well-known functions for the infant, it is necessary to separate it from the rest of the oligosaccharides to divide them into less complex fractions and analyse their partial unknown functions. For separation of lactose from HMOS two different stationary phases (size-exclusion gel as well as ion-exchange gel) were used. As the main result, it is shown that a size-exclusion gel with the particle size of 50-100 microm and porosity of 50 A was the preferred stationary phase for our separation process with almost complete lactose separation and stable conditions.

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Author

Andreas Geisser; Tatjana Hendrich; Günther Boehm; Bernd Stahl

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2005

Language

EN

Field

Chemistry (Physical Sciences)