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Understanding Spectrophotometry Basics by ScribdTranslations is a document available to read on EtoBox.
Spectrophotometry measures the absorption of light by substances. It is based on the Beer-Lambert laws, which state that absorption depends on the concentration of the substance and the length of the optical path. The spectrophotometer separates light into wavelengths and measures the absorption at each one, allowing the identification of substances by their characteristic absorption spectra. It has important applications in clinical biochemistry for the analysis of parameters.
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