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Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris by Christoph Sygmund; Petra Staudigl; Miriam Klausberger; Nikos Pinotsis; Kristina Djinović-Carugo; Lo Gorton; Dietmar Haltrich; Roland Ludwig is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris about?
## Background FAD dependent glucose dehydrogenase (GDH) currently raises enormous interest in the field of glucose biosensors. Due to its superior properties such as high turnover rate, substrate specificity and oxygen independence, GDH makes its way into glucose biosensing. The recently discovered GDH from the ascomycete __Glomerella cingulata__ is a novel candidate for such an electrochemical application, but also of interest to study the plant-pathogen interaction of a family of wide-spread, crop destroying fungi. Heterologous expression is a necessity to facilitate the production of GDH for biotechnological applications and to study its physiological role in the outbreak of anthracnose caused by __Glomerella__ (__anamorph Colletotrichum) spp__. ## Results Heterologous expression of active __G. cingulata__ GDH has been achieved in both __Escherichia coli__ and __Pichia pastoris__, however, the expressed volumetric activity was about 4800-fold higher in __P. pastoris__. Expression in __E. coli__ resulted mainly in the formation of inclusion bodies and only after co-expression with molecular chaperones enzymatic activity was detected. The fed-batch cultivation of a __P. pastoris__
Who reads Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Christoph Sygmund; Petra Staudigl; Miriam Klausberger; Nikos Pinotsis; Kristina Djinović-Carugo; Lo Gorton; Dietmar Haltrich; Roland Ludwig
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2002-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1475-2859)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)