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Determination of Phytoestrogens in Traditional Medicinal Herbs Using Gas Chromatography–mass Spectrometry by Sang Hee Lee; Byung Hwa Jung; Sun Yeou Kim; Bong Chul Chung is a Medicine article available to read on EtoBox.

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Quantitative analytical methods for the 17 phytoestrogens containing isoflavonoids, lignans, and mycoestrogens in herbs were developed, and the amount of phytoestrogens was determined in 22 traditional medicinal herbs. The focus of this study was to simplify the purification procedure for removing many kinds of interferences in herbs, and to select adequate derivatization reagent for getting desirable selectivity and sensitivity in the quantitative determination of phytoestrogens. To satisfy these goals, we performed a solid-phase extraction with Oasis HLB cartridges following enzymatic and acidic hydrolysis, and we used the mixture of MSTFA/NH4I/DTE (1000:4:5, v/w/w) to form TMS derivatives of phytoestrogens. Overall recovery was more than 84% in all of the phytoestrogens, and the limit of quantification for phytoestrogens in herbs were set at 0.2 microg/g. Coefficient of variation percentages were in the range of 0.18-15.68% (within-day) and 0.23-16.61% (day-to-day), respectively. Most of the isoflavonoids and lignans were found in all of the herbs, but mycoestrogens were not detected at all. The Leguminosae family proved to be the richest source of isoflavonoids. Lignans such as

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Author

Sang Hee Lee; Byung Hwa Jung; Sun Yeou Kim; Bong Chul Chung

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0955-2863)

Published

2004

Language

EN

Field

Medicine (Health Sciences)