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Decreased levels of CD44 protein and mRNA in prostate carcinoma: Correlation with tumor grade and ploidy by Bhaskar V. S. Kallakury; Fan Yang; James Figge; Karen E. Smith; Sankar J. Kausik; Nelson J. Tacy; Hugh A. G. Fisher; Ronald Kaufman; Helen Figge; Jeffrey S. Ross is a Medicine article available to read on EtoBox.

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BACKGROUND. CD44, a transmembrane protein, is associated with cell-cell and cell-matrix interaction and with tumor growth and metastasis. Expression of both standard form and variant isoforms of CD44 protein has been associated with aggressive behavior and metastasis in various tumors, but has not been characterized in prostate adenocarcinoma (PAC). ## METHODS. The expression of CD44 standard (CD44s) and splice variant v3, v4/5, v6, v7/8, and v10 proteins were studied in 109 PACs and correlated with tumor grade, DNA ploidy, and mRNA levels. Monoclonal antibodies against the various CD44 proteins were applied to microwave irradiated, formalin fixed, paraffin embedded sections. The DNA content of the tumors was evaluated by the Feulgen method with the CAS200 Image Analyzer. Total RNA exhibiting 18s and 28s bands was derived from two benign prostatic tissues and 5 PACs exhibiting decreased levels of CD44 protein by immunohistochemistry. The RNA was analyzed with reverse transcriptase polymerase chain reaction using CD44 specific primers. ## RESULTS. The basal cells of the benign prostatic acini revealed uniform membranous staining for CD44s, v3, and v6 in 95-97% of cases. Similar stai

Who reads Decreased levels of CD44 protein and mRNA in prostate carcinoma: Correlation with tumor grade and ploidy?

Author

Bhaskar V. S. Kallakury; Fan Yang; James Figge; Karen E. Smith; Sankar J. Kausik; Nelson J. Tacy; Hugh A. G. Fisher; Ronald Kaufman; Helen Figge; Jeffrey S. Ross

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0008-543X)

Published

1996

Language

EN

Field

Medicine (Health Sciences)