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Can I read Endocytic and Exocytic Pathways of the Neuronal Secretory Process and Trans Synaptic Transfer of Wheat Germ Agglutinin-horseradish Peroxidase in Vivo on EtoBox?
Endocytic and Exocytic Pathways of the Neuronal Secretory Process and Trans Synaptic Transfer of Wheat Germ Agglutinin-horseradish Peroxidase in Vivo by Richard D. Broadwell; Brian J. Balin is a Neuroscience article available to read on EtoBox.
What is Endocytic and Exocytic Pathways of the Neuronal Secretory Process and Trans Synaptic Transfer of Wheat Germ Agglutinin-horseradish Peroxidase in Vivo about?
The lectin wheat germ agglutinin (WGA) conjugated to horseradish peroxidase (HRP) was employed to study the endocytic and exocytic pathways of the secretory process in neurons and the potential for trans-synaptic transfer of molecules within the CNS. WGA-HRP binds to surface membrane oligosaccharides and enters cells by adsorptive endocytosis. The lectin conjugate was administered intranasally or into the cerebral ventricles of mice; postinjection survival times ranged from 5 minutes to 6 days. Due to binding of the lectin to ependymal cells subsequent to an intraventricular injection, only select populations of neurons (i.e., hippocampal formation; paraventricular nuclei; midbrain raphe; VI, X, XI1 motor nuclei; among others) were exposed extracellularly to WGA-HRP and became labeled by retrograde axoplasmic transport from axon terminals or by direct cell body/ dendritic uptake. WGA-HRP delivered intranasally was endocytosed by first-order olfactory neurons and transported by anterograde axoplasmic flow to the terminal field within the glomerular layer of the main olfactory bulb; eventually perikarya of the mitral cell layer were labeled, presumably by anterograde trans-synaptic t
Who reads Endocytic and Exocytic Pathways of the Neuronal Secretory Process and Trans Synaptic Transfer of Wheat Germ Agglutinin-horseradish Peroxidase in Vivo?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Richard D. Broadwell; Brian J. Balin
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)
- Published
- 1985
- Language
- EN
- Field
- Neuroscience (Life Sciences)