Can I read MlyI Nicking Enzyme on EtoBox?
MlyI Nicking Enzyme by pulaskiluke is a document available to read on EtoBox.
What is MlyI Nicking Enzyme about?
The study demonstrates the conversion of the Type IIs endonuclease MlyI into a nicking enzyme (N.MlyI) by mutating specific residues that disrupt its dimerization, allowing it to cleave only one DNA strand. N.MlyI retains high specific activity and is capable of nicking DNA at a specific site, providing a potential alternative for DNA manipulation without the need for costly reagents. This research opens avenues for engineering other Type IIs endonucleases into nicking enzymes, enhancing their utility in mo
- Author
- pulaskiluke
- Language
- EN