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Can I read Effects of acclimation and assay temperature on outer- and inner-ring thyroxine and 3,5,3′-triiodo-L-thyronine deiodination by liver microsomes of rainbow trout, Oncorhynchus mykiss on EtoBox?
Effects of acclimation and assay temperature on outer- and inner-ring thyroxine and 3,5,3′-triiodo-L-thyronine deiodination by liver microsomes of rainbow trout, Oncorhynchus mykiss by Dr. C. E. Johnston; J. G. Eales is a Environmental Science article available to read on EtoBox.
What is Effects of acclimation and assay temperature on outer- and inner-ring thyroxine and 3,5,3′-triiodo-L-thyronine deiodination by liver microsomes of rainbow trout, Oncorhynchus mykiss about?
## Abstract We studied effects of temperature on the deiodinating enzymatic pathways governing hepatic production of 3,5,3′‐triiodo‐L‐thyronine (T~3~) from L‐thyroxine (T~4~) in rainbow trout. Kinetics of outer‐ring deiodination (ORD) and inner‐ring deiodination (IRD) of T~4~ and T~3~ were measured in vitro at 4°, 12°, and 18°C using liver microsomes of trout that were acclimated for 21 days at 4°, 13°, and 18°C and fed on temperature‐compensated food rations. For all temperatures the V~max~ (level of functional enzyme) and V~max~/K~m~ (index of enzyme efficiency) values for the T~3~‐generating T~4~ORD pathway greatly exceeded values for the T~3~‐degrading T~3~IRD pathway; T~4~IRD and T~3~ORD pathways were negligible. The main effects of temperature occurred between 4°C and 12°/13°C; T~4~ORD V~max~/K~m~ rose from 0.48 at 4°C to 1.62 at 12°/13°C and to 1.83 at 18°C. This response correlated with in vivo deiodination rates measured previously. T~4~ORD apparent K~m~ did not change over the temperature acclimation range, suggesting an absence of temperature‐induced isozymes. However, K~m~ was depressed at the assay temperature of 4°C, indicating a direct effect of temperature to enhanc
Who reads Effects of acclimation and assay temperature on outer- and inner-ring thyroxine and 3,5,3′-triiodo-L-thyronine deiodination by liver microsomes of rainbow trout, Oncorhynchus mykiss?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Dr. C. E. Johnston; J. G. Eales
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-104X)
- Published
- 1995
- Language
- EN
- Field
- Environmental Science (Life Sciences)