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Proteomic analysis of exoproteins expressed by enterotoxigenic ** Staphylococcus aureus ** strains by Gabriella Pocsfalvi; Giuseppina Cacace; Manuela Cuccurullo; Giovanna Serluca; Alida Sorrentino; Gitta Schlosser; Giuseppe Blaiotta; Antonio Malorni is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Proteomic analysis of exoproteins expressed by enterotoxigenic ** Staphylococcus aureus ** strains about?
## Abstract Pathogenic bacteria excrete a variety of virulence factors into extracellular medium and to the cell surface which have essential roles in the colonization and insurrection of the host cells, and thus reflect the degree of bacterial pathogenicity. For the exploration of virulence factors expressed in the secreted proteome fraction, different __Staphylococcus aureus__ strains were analyzed using gel‐based bottom‐up proteomic approach. A total of 119 distinct proteins were identified for the enterotoxin gene cluster (__egc__) negative and __seb__ gene positive __S. aureus__ American Type Culture Collection (ATCC) 14458 strain by the use of one‐ and 2‐DE based proteomics. Detailed analysis of enterotoxin region of the 2‐D map confirmed, beside the highly expressed staphylococcal enterotoxin B (SEB), the presence of enterotoxin‐like proteins SElK and SElQ previously predicted by genotyping (Sergeev et al.., __J. Clin. Microbiol__. 2004, __42__, 2134–2143). Exoprotein patterns at the late‐exponential (7 h) and stationary (24 h) phases of cellular growth show a high‐level similarity in this region. Comparative analysis of enterotoxin region of five __S. aureus__ strains inclu
Who reads Proteomic analysis of exoproteins expressed by enterotoxigenic ** Staphylococcus aureus ** strains?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gabriella Pocsfalvi; Giuseppina Cacace; Manuela Cuccurullo; Giovanna Serluca; Alida Sorrentino; Gitta Schlosser; Giuseppe Blaiotta; Antonio Malorni
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)