About this Environmental Science article

Cadmium-induced oxidative damage and protective effects of N-acetyl-l-cysteine against cadmium toxicity in Solanum nigrum L. by Xiaopeng Deng; Yan Xia; Wei Hu; Hongxiao Zhang; Zhenguo Shen is a Environmental Science article available to read on EtoBox.

The effects of cadmium (Cd) on the accumulation of hydrogen peroxide (H 2 O 2 ) and antioxidant enzyme activities in roots of Solanum nigrum L. and the role of N-acetyl-l-cysteine (NAC) as a cysteine (Cys) donor against Cd toxicity were investigated. Cd at 50 and 200 M significantly increased the contents of thiobarbituric acid-reactive substances (TBARS), the production of H 2 O 2 and superoxide anion (O 2 •-), and the activities of catalase, guaiacol peroxidase, ascorbate peroxidase, glutathione peroxidase (GSH-Px), glutathione reductase, and superoxide dismutase. Experiments with diphenylene iodonium as an inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and NaN 3 as an inhibitor of peroxidase showed that the major source of Cd-induced reactive oxygen species in the roots may include plasma membrane-bound NADPH oxidase and peroxidase. In addition, the effects of NAC on plant growth, antioxidant enzyme activity, and non-protein thiol content were analyzed. Under Cd stress, the addition of 500 M NAC decreased the contents of TBARS and production of H 2 O 2 and O 2 •-, but increased levels of Cys and reduced glutathione (GSH), phytochelatins, and activity of

Author

Xiaopeng Deng; Yan Xia; Wei Hu; Hongxiao Zhang; Zhenguo Shen

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0304-3894)

Published

2010

Language

EN

Field

Environmental Science (Physical Sciences)