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What is Understanding Native PAGE Techniques about?

Sample preparation for native PAGE requires consideration as the net charge of proteins is determined by pH in the absence of SDS. Only proteins with a net negative charge will migrate into a native PAGE gel, as most proteins have a slightly acidic or basic pI between -3 to 8. The major difference between native and SDS-PAGE is that native PAGE separates proteins based on both mass and structure, while SDS-PAGE separates based only on mass by imparting a uniform negative charge to proteins.

Author

beatrice cho ming xuan

Language

EN