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Anchorage-dependent Mammalian Cell Culture Using Polyurethane Foam as a New Substratum for Cell Attachment by Taku Matsushita; Masayoshi Ketayama; Ken-ichi Kamihata; Kazumori Funatsu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A n c h o r a g e -d e p e n d e n t m a m m a l i a n cells were cultivated at high cell density in a novel culture system using p o l y u r e t h a n e f o a m ( P U F ) as a s u b s t r a t u m for cell attachment. P U F has a m a c r o p o r o u s structure giving a high surface a r e a to v o l u m e ratio. M o n k e y kidney cells (Vero) a n d Chinese h a m s t e r ovary cells ( C H O -K1) a t t a c h e d to the internal surface of P U F a n d grew to a high cell density ( 1 . 0 4 x 108 c e l l s / c m 3 P U F a n d 3.5 × 107 c e l l s / c m 3 P U F , respectively) in P U F stationary cultures. In a d d i t i o n , we have d e s i g n e d a P U F -p a rt i d e p a c k e d -b e d culture system for high density mass cell culture. A m a x i m u m cell density o f 2.4 x 107 c e l l s / cm 3 culture vessel v o l u m e was o b t a i n e d in a p a c k e db e d culture o f Vero cells.

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Author

Taku Matsushita; Masayoshi Ketayama; Ken-ichi Kamihata; Kazumori Funatsu

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1432-0614)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)