Opening book details…
Can I read Rapid Procedure for Detection and Isolation of Large and Small Plasmids on EtoBox?
Rapid Procedure for Detection and Isolation of Large and Small Plasmids by C I Kado; S T Liu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Rapid Procedure for Detection and Isolation of Large and Small Plasmids about?
Procedures are described for the detection and isolation of plasmids of various sizes (2.6 to 350 megadaltons) that are harbored in species of Agrobacterium, Rhizobium, Escherichia, Salmonella, Erwinia, Pseudomonas, and Xanthomonas. The method utilized the molecular characteristics of covalently closed circular deoxyribonucleic acid (DNA) that is released from cells under conditions that denature chromosomal DNA by using alkaline sodium dodecyl sulfate (pH 12.6) at elevated temperatures. Proteins and cell debris were removed by extraction with phenol-chloroform. Under these conditions chromosomal DNA concentrations were reduced or eliminated. The clarified extract was used directly for electrophoretic analysis. These procedures also permitted the selective isolation of plasmid DNA that can be used directly in nick translation, restriction endonuclease analysis, transformation, and DNA cloning experiments.
Who reads Rapid Procedure for Detection and Isolation of Large and Small Plasmids?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- C I Kado; S T Liu
- Publisher
- American Society for Microbiology
- Published
- 1981
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)