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The study investigates how the positioning of Chi sites influences the RecBCD pathway in bacterial recombinational repair of double-strand breaks (DSBs). It reveals that the suppression of Chi sites in repeated ribosomal RNA loci prevents genomic rearrangements by limiting the initiation of duplications. Experimental results demonstrate that successful DNA synthesis during repair requires specific interactions between initiating strands and homologous sequences, ultimately affecting genomic stability.
- Author
- Camila Moro
- Language
- EN