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Can I read Flow Injection Analysis for Glucose and Urea with Enzyme Reactors and On-line Dialysis on EtoBox?
Flow Injection Analysis for Glucose and Urea with Enzyme Reactors and On-line Dialysis by L. Gorton; L. Ögren is a Chemistry article available to read on EtoBox.
What is Flow Injection Analysis for Glucose and Urea with Enzyme Reactors and On-line Dialysis about?
A flow injection system for glucose and urea determination is described. The glucose determination uses immobilized glucose oxidase in a reactor designed to give 100% substrate conversion\_ The hydrogen peroxide formed is converted to a coloured complex with 4arninophenazone and ~,~~imethyl~iline\_ Tbe coupling is catalysed by a reactor containing immobifized peroxidase. The coloured complex ia measured in a flow-through spectrophotometric cell. Urea ia converted to ammonia in a reactor with immobilized urease and detected with an ammonia gas membrane electrode. Proteins and other inbrfering species from serum samples are removed in an on-line dialyuer. Calibration curves are linear for glucose in the range 1.6 X lo+-1.6 X lo-' M and for urea in the range lo-'-10" M. The sampies are 25 ~1 for glucose determination and 100 JII for urea determination. Linear ranges can be cbknged by varying the sample sizes. The effects of the dialyser, enzyme reactors and detectors on dispersion are evaluated. Continuous flow-injection analysis (f-i-a.
Who reads Flow Injection Analysis for Glucose and Urea with Enzyme Reactors and On-line Dialysis?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- L. Gorton; L. Ögren
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 1981
- Language
- EN
- Field
- Chemistry (Physical Sciences)