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Optimization suitable conditions for preparative isolation and separation of curculigoside and curculigoside B from Curculigo orchioides by high-speed counter-current chromatography by Jinyong Peng; Yunyun Jiang; Guorong Fan; Bin Chen; Qiaoyan Zhang; Yifeng Chai; Yutian Wu is a Engineering article available to read on EtoBox.
What is Optimization suitable conditions for preparative isolation and separation of curculigoside and curculigoside B from Curculigo orchioides by high-speed counter-current chromatography about?
A preparative high-speed counter-current chromatography (HSCCC) was used to isolate and separate curculigoside and curculigoside B from the famous traditional Chinese medicinal herb Curculigo orchioides. Some parameters including two-phase solvent system, separation temperature, flow rate of the mobile phase and revolution speed of the apparatus were all investigated, and a successfully isolation and purification was achieved at the following conditions: the two-phase solvent system composed of ethyl acetate-ethanol-water at a volume ratio of 5:1:5 (v/v/v) was selected and the lower phase of the system was used as the mobile phase at the flow rate of 2.0 ml/min, and the isolation temperature and revolution speed were set at 30 • C and 800 rpm. The isolation produced a total of 14.5 mg curculigoside B and 72.8 mg curculigoside with purities of 96.5% and 99.4% determined by high performance liquid chromatography (HPLC) from 300 mg crude extract after cleaning-up by D101 macroporous resin, which was necessary for the excellent purification. The recoveries of the two compounds were 91.6% and 92.5%, respectively, and the chemical structure identification was carried out by UV, MS and NM
Who reads Optimization suitable conditions for preparative isolation and separation of curculigoside and curculigoside B from Curculigo orchioides by high-speed counter-current chromatography?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Jinyong Peng; Yunyun Jiang; Guorong Fan; Bin Chen; Qiaoyan Zhang; Yifeng Chai; Yutian Wu
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 1383-5866)
- Published
- 2006
- Language
- EN
- Field
- Engineering (Physical Sciences)