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The catalytically inactive precursor of cathepsin D induces apoptosis in human fibroblasts and HeLa cells by Olga Schestkowa; Dominik Geisel; Ralf Jacob; Andrej Hasilik is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract In several reports cathepsin D has been implicated in apoptosis. In some systems the effects of agents considered to be mediated by cathepsin D were inhibited in the presence of pepstatin A, an inhibitor of the enzyme. In other studies the effect of a mutant cathepsin D deprived of activity was indistinguishable from that of the normal enzyme. Here we show that in human fibroblasts and in HeLa cells apoptosis can be induced by microinjecting into cytosol either mature cathepsin D or its inactive precursor procathepsin D. The microinjected precursor remains in the uncleaved form. These results confirm that the proapoptotic effect of cathepsin D in the cytosol is independent of its catalytic activity and suggest that the interaction of cathepsin D with the downstream effector does not involve the active site of the enzyme, since in the proenzyme the active site is masked by the prosequence. J. Cell. Biochem. J. Cell. Biochem. 101: 1558–1566, 2007. © 2007 Wiley‐Liss, Inc.

Who reads The catalytically inactive precursor of cathepsin D induces apoptosis in human fibroblasts and HeLa cells?

Author

Olga Schestkowa; Dominik Geisel; Ralf Jacob; Andrej Hasilik

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)