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Development of a Commercial Amperometric Biosensor Electrode for the Ketone D-3-hydroxybutyrate by Nigel J. Forrow; Gurdial S. Sanghera; Stephen J. Walters; Jared L. Watkin is a Engineering article available to read on EtoBox.

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Representatives of the common classes of quinoid NADH redox mediator, including Meldola Blue (MB) 3, 4-methyl-1,2-benzoquinone (4-MBQ) 4, 1-methoxy phenazine methosulphate (1-MeO-PMS) 5 and 2,6-dichloroindophenol (DCIP) 6, are shown to inhibit the NADdependent enzyme d-3-hydroxybutyrate dehydrogenase (HBDH), severely limiting their utility in the construction of a stable biosensor electrode for the ketone body d-3-hydroxybutyrate (3-OHB). It is proposed that these mediators bind covalently to important thiol groups in the enzyme. This mode of inhibition is overcome through the use of mediators such as 1,10-phenanthroline quinone (1,10-PQ) 7, which avoid 1,4-nucleophilic addition with enzyme amino acid residues such as Cys. As a result, 1,10-PQ 7 was selected for incorporation in a biosensor electrode for 3-OHB. The resulting MediSense ® Optium TM ␤-Ketone electrode is stable (≤10% loss in response at 30 • C versus 4 • C) with a long shelf life of 18 months. Diabetics can determine their d-3-hydroxybutyrate level with good precision (0.43 mM 3-OHB, 10.5% CV; 1.08 mM, 5.9%; 3.55 mM, 3.2%; n = 20 per level) and accuracy (versus reference assay: slope = 0.98; intercept = 0.02 mM, r = 0

Who reads Development of a Commercial Amperometric Biosensor Electrode for the Ketone D-3-hydroxybutyrate?

Author

Nigel J. Forrow; Gurdial S. Sanghera; Stephen J. Walters; Jared L. Watkin

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2005

Language

EN

Field

Engineering (Physical Sciences)