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RNA isolation from plant tissues rich in polysaccharides by Arun Dev Sharma; Prabhjot Kaur Gill; Prabhjeet Singh is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is RNA isolation from plant tissues rich in polysaccharides about?

Extraction of high-quality RNA is necessary for making cDNA libraries, isolating genes by RT-PCR, or investigating gene expression profiles. Several methods are commonly used for isolation of total RNA [1-3] and are being developed because plants contain high amounts of many different substances; therefore, just one nucleic acid isolation method suitable for all plants can never exist [4]. Even plant species belonging to the same genus or related genera can exhibit enormous variability in the complexity of pathways of dispensable functions. Thus, the biochemical compositions in plant tissues of different species are expected to vary considerably. The chemotypic heterogeneity among species may not allow optimal RNA yield from one isolation protocol and, perhaps, even closely related species may require different isolation protocols [5]. As our aim has been to study drought-induced differential expression of genes during early and late seed developmental stages in sorghum, we may need a protocol that not only can give the same quality and quantity of RNA at each stage but also is high yielding in order not to miss the rarely expressed genes. Previous standard plant RNA isolation proc

Who reads RNA isolation from plant tissues rich in polysaccharides?

Author

Arun Dev Sharma; Prabhjot Kaur Gill; Prabhjeet Singh

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)