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Betanine Separation And Quantification By Chromatography On Gels by J. P. ADAMS; J. H. von ELBE is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Betanine Separation And Quantification By Chromatography On Gels about?
## ABSTRACT Published methods for separation and quantification of betalaine pigments from plant extracts are based on electrophoretic and spectro‐photometric techniques. These methods are either time consuming, or lack accuracy if interfering substances are present; therefore, a more rapid and accurate method for betalaine separation and betanine quantification is needed. Chromatography on gel filtration supports (Sephadex or Bio‐Gel polyacrylamide) is shown to be a rapid and efficient method of separating betalaines from raw beet juice. Raw and fermented beet juice (__Beta vulgaris__) directly applied to a column of polyacrylamide gel (Bio‐Gel P‐6) resulted in the detection of numerous pigment bands. Observed average distribution coefficients (K~av~) for betanine on Sephadex G‐25 or Bio‐Gel P‐6 ranged from 0.8–2.0 at a pH value of 4.0–2.0, respectively. These data suggest that the major mechanism of retention of the pigments on gel supports can be adsorption rather than gel‐filtration. Resolution of betanine and betanidme was greater on columns packed with Sephadex G‐25 compared to columns packed with Bio‐Gel P‐6. Loading capacity (based on reduced plate height) was greater on co
Who reads Betanine Separation And Quantification By Chromatography On Gels?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- J. P. ADAMS; J. H. von ELBE
- Publisher
- Wiley
- Published
- 1977
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)